A Click Away From Choosing Key Elements In Consultant Interview For Medicine

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“Our findings are promising and indicate that we may have a path forward for the first time to treat the severe, life-limiting features of this genetic disorder,” said Yong-hui Jiang, M.D., Ph.D., associate professor in Duke’s departments of Pediatrics and Neurobiology. Jiang is senior author of a study published online Dec. 26 in the journal Nature Medicine. In most cases of Prader-Willi syndrome, the responsible gene in the region of chromosome 15 from the father is missing and the mother’s copy is silent. Jiang and colleagues focused their work on finding a way to activate the silent gene from the mother’s chromosome to recover the necessary gene function that would ordinarily be performed by the father’s gene. The researchers — including Bryan Roth, M.D., Ph.D, at the University of North Carolina at Chapel Hill and co-first authors Yuna Kim, Ph.D., and Hyeong-min Lee, Ph.D. — conducted screenings of more than 9,000 compounds. Using fluorescent marker in mouse embryonic fibroblasts, the researchers were able to see whether any of the small molecules triggered the cells to glow, which indicated they were capable of activating the maternal copy of the Prader-Willi gene. A class of small molecule that are known as G9a inhibitors were successful, both in the mouse model of Prader-Willi syndrome and in human cells from patients with the disorder. G9a is an enzyme that is important for gene regulation. The G9a inhibitors also appeared to have a therapeutic effect.

For the original version including any supplementary images or video, visit https://www.eurekalert.org/pub_releases/2016-12/dumc-pda122216.php

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